Kusunga umuna

Njira iyi yosungiramo umuna (ejaculate), monga cryopreservation, yayamba kwambiri mu cryomedicine. Zimaphatikizapo Kuwonjezera kwa sing'anga wapadera mpaka kumadzimadzi, ndikupitiriza kuzizira ndi kugwiritsa ntchito madzi a nayitrogeni. Ganizirani njira iyi yosungira maselo aamuna ammimba mwatsatanetsatane ndikukuwuzani kuti umuna uli woyenera kusamba ndi kusungiranso.

Kodi cryopreservation imawonjezera bwanji mwayi wa umuna?

Ndi feteleza zachilengedwe, chinsinsi cha glands zogonana chimasakanikirana pang'onopang'ono ndi maselo a epidermis, motero, kusintha kwina pa pH ya sing'anga kumachitika, zomwe zimayambitsa kuwonongedwa kwa lipoprotein chivundikiro cha spermatozoa, i.e. kuchitapo kanthu. Pokhala mu chikhalidwe ichi, nthawi ya moyo ya maselo obereka amakhala ochepa kwambiri, osaphatikizapo kugwiritsa ntchito kwawo kwa IVF. Ndichifukwa chake njira yogwiritsiridwa ntchito ikugwiritsidwa ntchito .

Njirayi imachulukitsa kwambiri masamu a umuna, ndipo imalola:

• kuchepetsa spermatozoa;
• amawapatsa iwo mphamvu yowonjezera;
• imateteza kachilombo ka HIV kuti isawonongeke;
• imalepheretsa kuwonjezeka kwa mankhwala opangidwa ndi poizoni;
• imalepheretsa kubereka kwa tizilombo toyambitsa matenda mu ejaculate.

Kodi amithenga ati amagwiritsidwa ntchito kusunga nyemba?

Pofuna kuthandiza, zomwe zimatchulidwa kuti zowonongeka ndi ejaculate, zofunikira kuti kusungulumwa kwa majeremusi, kuchotsa umuna. Zigwiritsiro zapadera zimagwiritsidwa ntchito.

Mpaka pano, ndizozoloŵera kugwiritsa ntchito zowonongeka zomwe zimasungidwa kuti zisungidwe, zomwe zimakhala zofunikira. Kawirikawiri, malo amtundu uwu ali ndi zigawo zingapo, makamaka osachepera atatu. Choncho, mu maonekedwe awo n'zotheka kupereka shuga, nthawi zambiri ndipo amagwiritsidwa ntchito shuga ndi lactose, sodium citrate.

Ngati tikulankhula za mankhwala omwe angagwiritsidwe ntchito ngati media kuti asunge umuna, ndiye kuti mwazinthu izi zimatchedwa Tris-Tffer, Trilon B, EDTA, Spermosan PPK.

Kodi ndi zinthu ziti zomwe ejaculate zasungidwa?

Njira yamakono yoziziritsa ndi kusunga umuna imayenera kugwirizanitsa ndi dongosolo lapadera lozizira, komanso kugwiritsa ntchito zipangizo zamakono. Pachifukwa ichi, chipinda cha ma laboratory chokha chiyenera kukhala ndi zipangizo zomwe zimapangitsa kuti mpweya uzizizira.

Musanayambe kusonkhanitsa ejaculate, zipangizo zonse zoyenera kupangidwira, makamaka mitundu yonse ya mabotolo, mapuloteni osungunuka, pipettes, zojambula zamapepala zimatetezedwa mu chipinda chapadera, kutentha kwa madigiri 130-150. Asanayambe ndondomeko yachindunji, iwo amaikidwa mu chipangizo chapadera, chomwe chimasunga kutentha kwa madigiri 37.

Kamuna kamuna kamatengedwa, imayikidwa mu botolo lopanda kanthu. Kutentha kosungirako umuna kumakhala nthawi zonse. Kuzizira kumachitika mu magawo awiri.

Pa yoyamba, ejaculate imayikidwa koyamba m'chipinda chozizizira, komwe kutentha kwake kumatsika pang'onopang'ono. Monga lamulo, mtengo wake ndi madigiri -35. Pambuyo pake, kuzizira kwakukulu kumachitika, kumiza botolo lapadera ndi umuna mu madzi amchere. Mkhalidwe umenewu, masamu a umuna amatha kufika zaka makumi angapo.

Kuti agwiritse ntchito ejaculate, yosungunuka kale, chotengera pamodzi ndi icho chimayikidwa m'madzi ofunda, kumene kuchepa kwapang'onopang'ono kumachitika. Pambuyo pake, cryoprotectants amagwiritsidwa ntchito, mwa kubwezeretsedwa mobwerezabwereza kwa umuna pa centrifuge. Pambuyo pake, madzi amadzimadzimwini amalowetsedwa ndi mchere wokhala ndi zakudya zam'mimba momwe amawukamo spermatozoa.